Human/Mouse S100A6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4584
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Immunohistochemistry, Plasma Culture, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse S100A6
Met1-Lys89
Accession # P14069
Met1-Lys89
Accession # P14069
Specificity
Detects human and mouse S100A6 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse S100A1 and recombinant mouse S100A4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse S100A6 Antibody
Detection of Mouse S100A6 by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/ Mouse S100A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4584) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A6 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
S100A6 in Human Kidney.
S100A6 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Mouse S100A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4584) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse S100A6 by Simple WesternTM.
Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for S100A6 at approximately 7 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse S100A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4584) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse S100A6 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Knockout Validated
S100A6
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
S100A6 knockout HEK293T cell line.
Simple Western
10 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Sample: NIH‑3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Western Blot
1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line and NIH-3T3 mouse embryonic fibroblast cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line and NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A6
Mouse S100A6 (also prolactin receptor-associated protein and calcyclin) is a 10 kDa member of the S100 family of calcium-binding proteins. S100A6 is 89 amino acids (aa) in length and contains two calcium-binding EF-hand domains (aa 12‑47 and 48‑83). Intracellularly, S100A6 will both noncovalently homodimerize and heterodimerize with S100B plus SGT1. Extracellularly, it is secreted via a noncanonical pathway and binds to RAGE, inducing apoptosis. It is expressed by neurons, endothelium, fibroblasts and glandular epithelia. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively.
Long Name
S100 Calcium Binding Protein A6
Alternate Names
CACY, MLN 4, PRA
Gene Symbol
S100A6
UniProt
Additional S100A6 Products
Product Documents for Human/Mouse S100A6 Antibody
Product Specific Notices for Human/Mouse S100A6 Antibody
For research use only
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