Human/Mouse SATB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8075
Key Product Details
Species Reactivity
Human, Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 815427
Product Specifications
Immunogen
E. coli-derived recombinant human SATB1
Met1-Ser260
Accession # Q01826
Met1-Ser260
Accession # Q01826
Specificity
Detects human and mouse SATB1 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse SATB1 Antibody
Detection of Human SATB1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SATB1 Monoclonal Antibody (Catalog # MAB8075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SATB1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SATB1 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for SATB1 at approximately 113 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse SATB1 Monoclonal Antibody (Catalog # MAB8075) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse SATB1 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SATB1
Long Name
SATB Homeobox 1
Alternate Names
DNA-binding protein SATB1, SATB homeobox 1, special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's), Special AT-rich sequence-binding protein 1
Gene Symbol
SATB1
UniProt
Additional SATB1 Products
Product Documents for Human/Mouse SATB1 Antibody
Product Specific Notices for Human/Mouse SATB1 Antibody
For research use only
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