Human/Mouse Serum Albumin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3329
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse Serum Albumin
Specificity
Detects human and mouse Serum Albumin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Serum Albumin Antibody
Detection of Human and Mouse Serum Albumin by Western Blot.
Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serum Albumin in HepG2 Human Cell Line.
Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human and Mouse Albumin by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Serum Albumin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Simple Western
5-10 µg/mL
Sample: Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum
Sample: Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum
Western Blot
0.1 µg/mL
Sample: Human liver tissue and mouse liver tissue
Sample: Human liver tissue and mouse liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Albumin
Additional Albumin Products
Product Documents for Human/Mouse Serum Albumin Antibody
Product Specific Notices for Human/Mouse Serum Albumin Antibody
For research use only
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