Human/Mouse Serum Albumin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3329

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF3329
AF3329-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunocytochemistry, Simple Western, Western Blot

Cited:

Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Albumin Antibodies »

Product Specifications

Immunogen

Mouse Serum Albumin

Specificity

Detects human and mouse Serum Albumin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Serum Albumin Antibody

Detection of Human and Mouse Serum Albumin antibody by Western Blot.

Detection of Human and Mouse Serum Albumin by Western Blot.

Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serum Albumin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Serum Albumin in HepG2 Human Cell Line.

Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human and Mouse Albumin antibody by Simple WesternTM.

Detection of Human and Mouse Albumin by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
View 2 more images

Applications for Human/Mouse Serum Albumin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Simple Western

5-10 µg/mL
Sample: Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum

Western Blot

0.1 µg/mL
Sample: Human liver tissue and mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Alternate Names

ALB

Entrez Gene IDs

213 (Human); 11657 (Mouse); 24186 (Rat)

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Human/Mouse Serum Albumin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Serum Albumin Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.