Human/Mouse SLAIN1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5638
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse SLAIN1
Met262-Gln483
Accession # Q68FF7
Met262-Gln483
Accession # Q68FF7
Specificity
Detects human and mouse SLAIN1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse SLAIN1 Antibody
Detection of Human/Mouse SLAIN1 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SLAIN1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SLAIN1 in D3 Mouse Cell Line.
SLAIN1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse SLAIN1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line
Sample: Immersion fixed D3 mouse embryonic stem cell line
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells
Sample: C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLAIN1
Long Name
Slain motif-containing Protein 1
Alternate Names
SLAIN motif family, member 1
Gene Symbol
SLAIN1
UniProt
Additional SLAIN1 Products
Product Documents for Human/Mouse SLAIN1 Antibody
Product Specific Notices for Human/Mouse SLAIN1 Antibody
For research use only
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