Human/Mouse SMOC-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5140
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Functional Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SMOC-2
Gln22-Gly446
Accession # Q9H3U7
Gln22-Gly446
Accession # Q9H3U7
Specificity
Detects human and mouse SMOC‑2 in direct ELISAs and Western Blots
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse SMOC-2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human SMOC‑2 (Catalog # 5140-SM)
Recombinant Mouse SMOC‑2 (Catalog # 6075-SM)
Sample: Recombinant Human SMOC‑2 (Catalog # 5140-SM)
Recombinant Mouse SMOC‑2 (Catalog # 6075-SM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMOC-2
SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins. It is widely expressed, perhaps by endothelium and apparently participates in both growth factor signaling and ECM organization. Mature human SMOC-2 is 425 amino acids (aa) in length. It contains one Kazal-like domain (aa 40‑84), two thyroglobulin type-1 segments (aa 87‑153 and 213‑281) and two EF-hand sequences (aa 347‑382 and 384‑419). There are three splice variants. One shows a 13 aa substitution for aa 442‑446, another shows an 11 aa insertion after Thr170 and a third represents a combination of the above two variations. Over aa 22‑446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.
Long Name
Secreted Modular Calcium-binding Protein 2
Alternate Names
MST117, MSTP117, MSTP140, SMAP2, SMOC2
Gene Symbol
SMOC2
UniProt
Additional SMOC-2 Products
Product Documents for Human/Mouse SMOC-2 Antibody
Product Specific Notices for Human/Mouse SMOC-2 Antibody
For research use only
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