Human/Mouse Somatostatin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2358
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 906552
Product Specifications
Immunogen
KLH-coupled human Somatostatin peptide
Accession # P61278
Accession # P61278
Specificity
Detects human Somatostatin in direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human/Mouse Somatostatin Antibody
Somatostatin in Human Embryonic Stem Cells.
Somatostatin was detected in immersion fixed human embryonic stem cells differentiated to pancreatic cells using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Somatostatin in betaTC‑6 Mouse Cell Line.
Somatostatin was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Somatostatin in Human Pancreas.
Somatostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse Somatostatin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human embryonic stem cells differentiated into pancreatic cells and betaTC‑6 mouse beta cell insulinoma cell line, TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)
Sample: Immersion fixed human embryonic stem cells differentiated into pancreatic cells and betaTC‑6 mouse beta cell insulinoma cell line, TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Reviewed Applications
Read 2 reviews rated 5 using MAB2358 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Somatostatin
Somatostatin (SST), also known as SMST, is a 116 amino acid (aa) peptide hormone protein and shares 96% aa identity with mouse and rat SST. It exists as a preproprotein that is cleaved into a proprotein form and then subsequently into 14 aa and 28 aa active peptide forms. SST is secreted by many tissues throughout the body and inhibits the secretion of many other hormones. Some of its major roles are inhibiting growth hormone secretion by the pituitary gland, insulin and glucagon secretion in pancreatic islets, and secretion of gastrointestinal tract hormones such as gastrin. Additionally, SST has been shown to have neuromodulatory activity.
Alternate Names
SMST, SOM, SRIF, SST
Gene Symbol
SST
UniProt
Additional Somatostatin Products
Product Documents for Human/Mouse Somatostatin Antibody
Product Specific Notices for Human/Mouse Somatostatin Antibody
For research use only
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