Human/Mouse SSEA-4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1435
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Product Specifications
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse SSEA-4 Biotinylated Antibody
SSEA-4 in BG01V Human Stem Cells.
SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Biotinylated Monoclonal Antibody (Catalog # BAM1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse SSEA-4 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: NTera-2 human testicular embryonic carcinoma cell line
Sample: NTera-2 human testicular embryonic carcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001)
Sample: Immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001)
Reviewed Applications
Read 1 review rated 5 using BAM1435 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSEA-4
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
References
- Shevinsky, L.H. et al. (1982) Cell 30:697.
- Kannagi, R. et al. (1983) EMBO J. 2:2355.
- Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
- Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for Human/Mouse SSEA-4 Biotinylated Antibody
Product Specific Notices for Human/Mouse SSEA-4 Biotinylated Antibody
For research use only
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