Human/Mouse TBX5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5918

R&D Systems, part of Bio-Techne
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AF5918
AF5918-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all TBX5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX5
Lys253-Lys327
Accession # Q99593

Specificity

Detects human and mouse TBX5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse TBX5 Antibody

Detection of Human and Mouse TBX5 antibody by Western Blot.

Detection of Human and Mouse TBX5 by Western Blot.

Western blot shows lysates of human heart and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TBX5 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human and Mouse TBX5 antibody by Simple WesternTM.

Detection of Human and Mouse TBX5 by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for TBX5 at approximately 56 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human/Mouse TBX5 Antibody

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: Human heart tissue and mouse heart tissue

Western Blot

1 µg/mL
Sample: Human heart and mouse embryonic heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX5

TBX5 (T-box protein 5) is a 64 kDa member of the T-box family of transcriptional regulators. It is expressed in both embryo and adult, and is particularly important in the development of the heart and upper limb. TBX5 is a transcriptional activator and influences the expression of FGF‑10 and NPPA/ANF. Human TBX5 is 518 amino acids (aa) in length. It contains one T‑box DNA‑binding domain (aa 58‑238), a transactivation motif (Glu349GluAsp351), and an NLS (aa 339‑379). There are multiple splice forms. One 35 kDa isoform shows a RPLL substitution for aa 252‑518, generating a TBX5 antagonist. Other isoforms show an alternative start site at Met51, a 136 aa substitution for aa 445‑518, a 33 aa substitution for aa 360‑518, a 22 aa substitution for aa 328‑518, and a 41 aa substitution for aa 7‑49. Over aa 253‑327, human TBX5 shares 95% aa identity with mouse TBX5.

Long Name

T-box Transcription Factor 5

Alternate Names

HOS, T-box 5, T-box protein 5, T-box transcription factor TBX5

Entrez Gene IDs

6910 (Human); 21388 (Mouse)

Gene Symbol

TBX5

UniProt

Q99593

Additional TBX5 Products

Product Documents for Human/Mouse TBX5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TBX5 Antibody

For research use only

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