Human/Mouse THAP11 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5727

R&D Systems, part of Bio-Techne
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AF5727
AF5727-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all THAP11 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human THAP11
Gly226-Met314
Accession # Q96EK4

Specificity

Detects human and mouse THAP11 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse THAP11 Antibody

Detection of Human THAP11 antibody by Western Blot.

Detection of Human THAP11 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human THAP11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5727) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for THAP11 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse THAP11 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THAP11

THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.

Long Name

THAP Domain Containing 11

Alternate Names

CTG-B43a, CTG-B45d, Ronin

Entrez Gene IDs

57215 (Human); 59016 (Mouse); 307806 (Rat)

Gene Symbol

THAP11

UniProt

Q96EK4

Additional THAP11 Products

Product Documents for Human/Mouse THAP11 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse THAP11 Antibody

For research use only

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