Human/Mouse TLE3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5785

R&D Systems, part of Bio-Techne
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AF5785
AF5785-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TLE3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TLE3
His11-Asn200
Accession # Q04726

Specificity

Detects endogenous human TLE3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse TLE3 Antibody

Detection of Human TLE3 antibody by Western Blot.

Detection of Human TLE3 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse TLE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TLE3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse TLE3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MCF-7 human breast cancer cell line and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLE3

TLE3 (Transducin-Like Enhancer of Split 3; also ESG3 and GRG3) is an 84‑86 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed in the embryo, and in differentiating epithelial cells in the adult. TLE3 binds to a number of transcription factors as a heterooligomer, or to itself as a homotetramer. Oligomerization is essential for activity. Human TLE3is 772 amino acids (aa) in length. It contains a Gln(Q)-rich region that mediates oligomerization (aa 1‑131), a CCN domain that contains an NLS plus CKII and cdc2K kinase site (aa 199‑268), and seven WD repeats that mediate protein-protein interaction (aa 484‑771). Potentially there are multiple splice variants. Over aa 11‑200, human TLE3shares 99% aa identity wih mouse TLE3.

Long Name

Transducin-like Enhancer of Split 3

Alternate Names

ESG3, Grg3

Entrez Gene IDs

7090 (Human); 21887 (Mouse); 84424 (Rat)

Gene Symbol

TLE3

UniProt

Q04726

Additional TLE3 Products

Product Documents for Human/Mouse TLE3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TLE3 Antibody

For research use only

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