Human/Mouse TRAP220 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5520
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TRAP220
Phe878-Phe1031
Accession # Q15648
Phe878-Phe1031
Accession # Q15648
Specificity
Detects endogenous human TRAP220 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse TRAP220 Antibody
Detection of Human TRAP220 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.Applications for Human/Mouse TRAP220 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 2 reviews rated 4 using AF5520 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAP220
TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.
Long Name
Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa
Alternate Names
DRIP230, MBD4, MED1, PPARBP, PPARGBP, TRIP2
Gene Symbol
MED1
UniProt
Additional TRAP220 Products
Product Documents for Human/Mouse TRAP220 Antibody
Product Specific Notices for Human/Mouse TRAP220 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...