Human/Mouse VGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6317
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 619707
Product Specifications
Immunogen
E. coli-derived recombinant human VGF
Thr554-Pro615
Accession # O15240
Thr554-Pro615
Accession # O15240
Specificity
Detects human and mouse VGF in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse VGF Antibody
VGF in Human Brain.
VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse VGF Antibody
Application
Recommended Usage
Immunohistochemistry
1.7-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and perfusion fixed frozen sections of mouse brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and perfusion fixed frozen sections of mouse brain (cortex)
Reviewed Applications
Read 1 review rated 5 using MAB6317 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VGF
Long Name
Neurosecretory Protein VGF
Alternate Names
neuro-endocrine specific protein VGF, neurosecretory protein VGF, VGF nerve growth factor inducible
Entrez Gene IDs
7425 (Human)
Gene Symbol
VGF
UniProt
Additional VGF Products
Product Documents for Human/Mouse VGF Antibody
Product Specific Notices for Human/Mouse VGF Antibody
For research use only
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