Human MRP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1929
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 464510
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human MPR1
Met1-Val1531
Accession # P33527
Met1-Val1531
Accession # P33527
Specificity
Detects human MRP1. Detects human MRP1-transfected cells, but not irrelevant transfectants by flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human MRP1 Antibody
Detection of MRP1 in Human Lymphocytes by Flow Cytometry.
Human blood-derived lymphocytes were stained with Mouse Anti-Human MRP1 Monoclonal Antibody (Catalog # MAB1929, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human MRP1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human blood-derived lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Sample: Human blood-derived lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MRP1
MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.
Long Name
Multi-drug Resistance Protein 1
Alternate Names
ABCC1, GS-X
Gene Symbol
ABCC1
UniProt
Additional MRP1 Products
Product Documents for Human MRP1 Antibody
Product Specific Notices for Human MRP1 Antibody
For research use only
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