Human MSX2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7917

R&D Systems, part of Bio-Techne
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MAB7917
MAB7917-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 786607

View all MSX2 Antibodies »

Product Summary for Human MSX2 Antibody

Immunogen

E. coli-derived recombinant human MSX2
Ala2-Thr132
Accession # P35548

Specificity

Detects human MSX2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MSX2 Antibody

MSX2 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC).

MSX2 in SK‑BR‑3 Human Cell Line.

MSX2 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human MSX2 Monoclonal Antibody (Catalog # MAB7917) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MSX2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SK‑BR‑3 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX2

MSX2, also known as HOX-8, is an approximately 35 kDa homeobox transcription factor that is a downstream target of canonical Wnt signaling. MSX2 plays an important role in the development of mineralized tissues including bone, cartilage, and teeth. It is upregulated in atherosclerotic plaques and promotes the calcification of vascular smooth muscle cells during inflammation. MSX2 suppresses the adipocytic differentiation of multipotent mesenchymal cells. MSX2 acts in mammary gland development and the initiation of female fetal germ cell meiosis. It promotes tumorigenesis of breast and pancreatic carcinomas but inhibits the survival and invasiveness of melanoma cells. Human MSX2 contains one homeodomain (aa 143-201). Within aa 1-132, human MSX2 shares 87% aa sequence identity with mouse and rat MSX2.

Long Name

Msh Homeobox 2

Alternate Names

CRS2, FPP, Hox8, MSH, PFM1

Entrez Gene IDs

4488 (Human); 17702 (Mouse); 25483 (Rat)

Gene Symbol

MSX2

UniProt

P35548 (Human)

Additional MSX2 Products

Product Documents for Human MSX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MSX2 Antibody

For research use only

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