MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC4 Monoclonal Antibody (Catalog # MAB8195, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).

Applications for Human MUC-4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: MCF‑7 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUC-4

MUC-4 (Mucin-4), also called ASGP (ascites sialoglycoprotein) is a highly glycosylated type I transmembrane glycoprotein that may be up to 950 kDa in its full-length, fully glycosylated form. Human MUC-4 cDNA encodes 2169 amino acids (aa) with a 28 aa signal sequence and a cleavage site that creates a 1416 aa soluble, extracellular alpha chain and a 725 aa single-pass transmembrane beta chain. Between aa 1072-1317 within the alpha chain, human MUC-4 shares 69% aa sequence identity with mouse and rat MUC-4. At least 14 soluble or transmembrane splice variants of 1102-2117 aa have been described, 5 of which contain the full sequence used as an immunogen. Muc-4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC-4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Entrez Gene IDs

4585 (Human); 140474 (Mouse)

Gene Symbol

MUC4

UniProt

Q99102

Additional MUC-4 Products

Product Documents for Human MUC-4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MUC-4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MUC-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8195

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all MUC-4 Antibodies »

Product Summary for Human MUC-4 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MUC-4 Antibody

Detection of MUC4 in MCF‑7 Human Cell Line by Flow Cytometry.