Human MUC-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8195
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MUC-4
Asn1137-Glu1317
Accession # Q99102
Asn1137-Glu1317
Accession # Q99102
Specificity
Detects human MUC-4 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MUC-4 Antibody
MUC‑4 in Human Stomach.
MUC-4 was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human MUC-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8195) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MUC-4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach
Sample: Immersion fixed paraffin-embedded sections of human stomach
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MUC-4
MUC-4 (Mucin-4), also called ASGP (Ascites Sialoglycoprotein) is a highly glycosylated type I transmembrane glycoprotein that may be up to 950 kDa in its full-length, fully glycosylated form. Human MUC-4 cDNA encodes 2169 amino acids (aa) with a 28 aa signal sequence and a cleavage site that creates a 1416 aa soluble, extracellular alpha chain and a 725 aa single-pass transmembrane beta chain. Between aa 1072-1317 within the alpha chain, human MUC-4 shares 69% aa sequence identity with mouse and rat MUC-4. At least 14 soluble or transmembrane splice variants of 1102-2117 aa have been described, 5 of which contain the full sequence used as an immunogen. MUC-4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC-4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.
Long Name
Mucin 4, Cell Surface-associated
Alternate Names
ASGP, MUC4
Gene Symbol
MUC4
UniProt
Additional MUC-4 Products
Product Documents for Human MUC-4 Antibody
Product Specific Notices for Human MUC-4 Antibody
For research use only
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