Human MUCDHL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3826
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MUCDHL
Gln26-Ala434
Accession # Q9HBB8
Gln26-Ala434
Accession # Q9HBB8
Specificity
Detects human MUCDHL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse MUCDHL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MUCDHL Antibody
MUCDHL in Human Kidney.
MUCDHL was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human MUCDHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3826) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in collecting tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MUCDHL Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
0.1 µg/mL
Sample: Recombinant Human MUCDHL
Sample: Recombinant Human MUCDHL
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MUCDHL
Mucin and cadherin-like protein (MUCDHL), also known as μ-protocadherin, is a 110 kDa type I transmembrane protein that is both O- and N-glycosylated. It is a calcium-dependant cell adhesion protein in the cadherin superfamily. MUCDHL is found on intestinal and renal epithelium. Human MUCDHL extracellular domain (ECD) contains four cadherin domains (residues 71-459) plus several mucin domains (residues 540-645). Alternate splicing creates four isoforms that differ in their mucin domains. In the region used as immunogen, human and mouse MUCDHL share 63% amino acid sequence identity.
Long Name
Mucin And Cadherin-like
Alternate Names
CDHR5, MU-PCDH, MUPCDH
Gene Symbol
CDHR5
UniProt
Additional MUCDHL Products
Product Documents for Human MUCDHL Antibody
Product Specific Notices for Human MUCDHL Antibody
For research use only
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