Rat cortical stem cells were stained with Mouse Anti-Human Musashi-2 Monoclonal Antibody (Catalog # MAB3255, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Musashi‑2 in K562 Human Cell Line.

Musashi-2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human Musashi-2 Monoclonal Antibody (Catalog # MAB3255) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Musashi-2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-2

Musashi-2 belongs to the evolutionarily conserved Musashi family of RNA binding proteins which are involved in the translational control of their target mRNAs. Musashi-2 has two tandem RNA-recognition domains (RRM-1 and RRM-2) that have the highly conserved RNP (ribonucleoprotein) motifs. By alternative splicing, at least three Musashi-2 isoforms exist. In mammalian nervous system, Musashi-1 and –2 are selectively expressed in neural progenitor cells and play important roles in maintenance of the stem cell fate. Human Musashi-2 shares 95% amino acid sequence homology with mouse Musashi-2 and 80% amino acid sequence identity with human Musashi-1. Translocations resulting in the formation of Musashi-2/HOXA9 fusion protein is associated with progression of chronic myelogenous leukemia to the accelerated phase and blast crisis.

Alternate Names

MSI2, Musashi2

Entrez Gene IDs

124540 (Human); 76626 (Mouse)

Gene Symbol

MSI2

UniProt

Q96DH6

Additional Musashi-2 Products

Product Documents for Human Musashi-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Musashi-2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Musashi-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3255

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Musashi-2 Antibodies »

Product Summary for Human Musashi-2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Musashi-2 Antibody

Detection of Musashi‑2 in Rat Cortical Stem Cells by Flow Cytometry.