Human Mxi1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4185
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Chromatin Immunoprecipitation Sequencing
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Mxi1
Ala81-Ser228
Accession # P50539
Ala81-Ser228
Accession # P50539
Specificity
Detects human Mxi1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) MAD1, rhMAD3, and rhMAD4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Mxi1 Antibody
Mxi1 in LNCaP Human Cell Line.
Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Mxi1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line and U937 human histiocytic lymphoma cell line treated with PMA
Sample: Immersion fixed LNCaP human prostate cancer cell line and U937 human histiocytic lymphoma cell line treated with PMA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4185 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mxi1
(aa 69‑80) and a bHLH domain (aa 81‑120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81‑228, human Mxi-1 is 95% aa identical to mouse Mxi-1.
Long Name
MAX Interacting Protein 1
Alternate Names
MAD2, MXD2, MXI, MXI-WR
Gene Symbol
MXI1
UniProt
Additional Mxi1 Products
Product Documents for Human Mxi1 Antibody
Product Specific Notices for Human Mxi1 Antibody
For research use only
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