Human Myeloperoxidase/MPO Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB31741
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 358621
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived human Myeloperoxidase/MPO protein
Ala49-Ser745
Accession # P05164
Ala49-Ser745
Accession # P05164
Specificity
Detects human Myeloperoxidase/MPO in sandwich ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Myeloperoxidase/MPO Antibody
Human Myeloperoxidase/MPO ELISA Standard Curve.
Recombinant Human Myeloperoxidase/MPO protein was serially diluted 2-fold and captured by Rat Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB31741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human Myeloperoxidase/MPO Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody.
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Myeloperoxidase DuoSet ELISA Kit (Catalog # DY3174) for convenient development of a sandwich ELISA or the Human Myeloperoxidase Quantikine ELISA Kit (Catalog # DMYE00B) for a complete optimized ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta-subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta-subunits and two alpha-subunits that are also disulfide-linked through the two alpha-subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.
Alternate Names
MPO
Gene Symbol
MPO
UniProt
Additional Myeloperoxidase/MPO Products
Product Documents for Human Myeloperoxidase/MPO Antibody
Product Specific Notices for Human Myeloperoxidase/MPO Antibody
For research use only
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