Human Myosin Heavy Chain Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC4470F

R&D Systems, part of Bio-Techne
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IC4470F
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # MF20

View all Myosin Heavy Chain Antibodies »

Product Specifications

Immunogen

Chicken pectoralis-derived Myosin

Specificity

Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Myosin Heavy Chain Fluorescein-conjugated Antibody

Detection of Myosin Heavy Chain antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of Myosin Heavy Chain in C2C12 Mouse Cell Line by Flow Cytometry.

Three-day differentiated C2C12 mouse myoblast cell line was stained with Mouse Anti-Human Myosin Heavy Chain Fluorescein-conjugated Monoclonal Antibody (Catalog # IC4470F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Myosin Heavy Chain Fluorescein-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Three-day differentiated C2C12 mouse myoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Myosin Heavy Chain

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Alternate Names

MYHC

Additional Myosin Heavy Chain Products

Product Documents for Human Myosin Heavy Chain Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Myosin Heavy Chain Fluorescein-conjugated Antibody

For research use only

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