Human N-Terminal Pro-BNP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9538
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 370129
Product Specifications
Immunogen
E. coli-derived recombinant human N-Terminal Pro-BNP
His27-His134
Accession # P16860
His27-His134
Accession # P16860
Specificity
Detects human N-Terminal Pro-BNP in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human N-Terminal Pro-BNP Antibody
Human NT Pro-BNP ELISA Standard Curve.
Recombinant human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9538) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Human NT Pro-BNP ELISA Standard Curve.
Recombinant Human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human N-Terminal Pro-BNP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human N-terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042).
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human NT Pro-BNP DuoSet ELISA Kit (Catalog # DY3604-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BNP
Long Name
Brain Natriuretic Peptide
Alternate Names
BNF, NPPB
Gene Symbol
NPPB
UniProt
Additional BNP Products
Product Documents for Human N-Terminal Pro-BNP Antibody
Product Specific Notices for Human N-Terminal Pro-BNP Antibody
For research use only
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