Human Nanos2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2870
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 343255
Product Specifications
Immunogen
E. coli-derived recombinant human Nanos2
Met1-Arg138
Accession # P60321
Met1-Arg138
Accession # P60321
Specificity
Detects human Nanos2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nanos1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Nanos2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Nanos2
Sample: Recombinant Human Nanos2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nanos2
Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos‑type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.
Alternate Names
nanos homolog 2, nanos homolog 2 (Drosophila), NOS2NOS-2
Entrez Gene IDs
339345 (Human)
Gene Symbol
NANOS2
UniProt
Additional Nanos2 Products
Product Documents for Human Nanos2 Antibody
Product Specific Notices for Human Nanos2 Antibody
For research use only
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