Human Neurogranin Antibody

Detection of Human, Mouse, and Rat Neurogranin by Western Blot.

Western blot shows lysates of human brain (motor cortex), mouse brain, and rat brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Neurogranin at approximately 14-15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Neurogranin in SW480 Human Cell Line .

Neurogranin was detected in immersion fixed SW480 human liposarcoma cell line (positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining is performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neurogranin in Human Brain (Cortex).

Neurogranin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.