Human Neurturin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB387
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Blockade of Receptor-ligand Interaction, Western Blot
Cited:
ELISA Development (Detection)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 126422
Product Specifications
Immunogen
E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748
Ala96-Val197
Accession # Q99748
Specificity
Detects human Neurturin in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Neurturin is observed and no cross-reactivity with recombinant mouse (rm) Artemin, recombinant human (rh) Cripto-1, recombinant Drosophila DPP, rhGDNF, rhLefty A, rmLefty-1, rhMIS, rmNodal, rmPersephin, rhTGF-beta 1, rhLatent TGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2 or rhTGF-beta 3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human Neurturin Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.15 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 1297-NE) to immobilized Recombinant Human GFR alpha-2 Fc Chimera (Catalog # 613‑FR) coated at 1 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
Western Blot
1 µg/mL
Sample: Recombinant Human Neurturin (Catalog # 1297-NE)
Sample: Recombinant Human Neurturin (Catalog # 1297-NE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurturin
Alternate Names
NRTN, NTN
Gene Symbol
NRTN
UniProt
Additional Neurturin Products
Product Documents for Human Neurturin Antibody
Product Specific Notices for Human Neurturin Antibody
For research use only
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