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Human Neurturin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3871

R&D Systems, part of Bio-Techne
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MAB3871
MAB3871-SP

Key Product Details

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction, ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 126424

Product Specifications

Immunogen

E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748

Specificity

Detects human Neurturin in direct ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant mouse Neurturin, recombinant human (rh) TGF-alpha, rhTGF-beta 2, rhTGF-beta 1.2, rhTGF-beta 3, rhGDNF, rhLAP, or recombinant rat TGF-beta 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Neurturin Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.06-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 1297-NE) to immobilized Recombinant Human GFR alpha-2 Fc Chimera (Catalog # 613‑FR) coated at 1 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Neurturin Monoclonal Antibody (Catalog # MAB3872).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

1 µg/mL
Sample: Recombinant Human Neurturin (Catalog # 1297-NE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands and is distantly related to TGF-beta family ligands. It is a disulfide-linked homodimer that interacts with a receptor complex composed of a high affinity ligand binding component (GFR alpha1 or GFR alpha2) and a common signaling component, cRET. Neurturin promotes the survival of a variety of neurons and possibly other cell types.

Alternate Names

NRTN, NTN

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Gene Symbol

NRTN

UniProt

Additional Neurturin Products

Product Documents for Human Neurturin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Neurturin Antibody

For research use only

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