Human NF-H Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3108
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NF-H
Met1-Ala380
Accession # P12036
Met1-Ala380
Accession # P12036
Specificity
Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) NF-M and rhNF-L is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NF-H Antibody
NF-H in Human Brain.
NF-H was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human NF-H by Simple WesternTM.
Simple Western shows lysates of human cerebellum, loaded at 0.2 mg/ml. A specific band was detected for NF-H at approximately 373 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.Applications for Human NF-H Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Simple Western
2.5 µg/mL
Sample: Human cerebellum
Sample: Human cerebellum
Western Blot
0.1 µg/mL
Sample: Human NF-H
Sample: Human NF-H
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-H
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Entrez Gene IDs
4744 (Human)
Gene Symbol
NEFH
UniProt
Additional NF-H Products
Product Documents for Human NF-H Antibody
Product Specific Notices for Human NF-H Antibody
For research use only
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