Human NF-L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB22165
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1033926
Product Specifications
Immunogen
Human NF-L synthetic peptide
Specificity
Detects human NF-L in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NF-L Antibody
Detection of NF-L in Human Hippocampus.
NF-L was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB22165) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human NF-L Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-L
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Entrez Gene IDs
4747 (Human)
Gene Symbol
NEFL
Additional NF-L Products
Product Documents for Human NF-L Antibody
Product Specific Notices for Human NF-L Antibody
For research use only
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