Human NF2/Merlin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5616

R&D Systems, part of Bio-Techne
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AF5616
AF5616-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all NF2/Merlin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF2
Met363-Lys578
Accession # P35240

Specificity

Detects human NF2 in Western blots and direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NF2/Merlin Antibody

NF2/Merlin antibody in Human Brain by Immunohistochemistry (IHC-P).

NF2/Merlin in Human Brain.

NF2/Merlin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human NF2/Merlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5616) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human NF2/Merlin antibody by Western Blot.

Detection of Human NF2/Merlin by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NF2/Merlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NF2/Merlin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NF2/Merlin antibody in SK-HEP-1 Human Cell Line by Immunocytochemistry (ICC).

NF2/Merlin in SK-HEP-1 Human Cell Line.

NF2/Merlin was detected in immersion fixed SK-HEP-1 human liver endothelial cell line using Goat Anti-Human NF2/Merlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5616) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human NF2/Merlin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SK-HEP-1 human liver endothelial cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cerebellum) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line and U2OS human osteosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF2/Merlin

NF2 (Neurofibromin-2; also Merlin) is a 70‑75 kDa tumor suppressor protein belonging to the ERM (ezrin, radixin, moesin) family of proteins. It participates in contact‑mediated cell adhesion, thereby blocking cell proliferation and migration. Human NF2 is 595 amino acids (aa) in length. It contains three general regions. There is an N‑terminal FERM domain (aa 1‑302), a alpha-helical rod central region (aa 303‑478), and a unique carboxy-terminal domain (aa 479‑595). Confluence requires an absence of phosphorylation at Ser516 and Ser10. There are multiple potential splice variants. Four short forms less that 260 aa in length lack the aa range used for antigen immunization. There are at least eight additional isoforms that basically show an alternate start site coupled to an 11 aa substitution for aa 579‑595. Lack of NF2 is associated with neurofibromatosis type II.

Long Name

Neurofibromin 2

Alternate Names

ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin

Entrez Gene IDs

4771 (Human); 18016 (Mouse); 25744 (Rat)

Gene Symbol

NF2

UniProt

P35240

Additional NF2/Merlin Products

Product Documents for Human NF2/Merlin Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NF2/Merlin Antibody

For research use only

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