Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 74902

View all NGFR/TNFRSF16 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
Lys29-Asn250
Accession # P08138

Specificity

Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NGFR/TNFRSF16 APC-conjugated Antibody

Detection of NGF R/TNFRSF16 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry.

Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 APC-conjugated Monoclonal Antibody (Catalog # FAB367A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NGFR/TNFRSF16 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: SH‑SY5Y human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 4 using FAB367A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: NGFR/TNFRSF16

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).

References

  1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
  2. Stemple, D.L. et al. (1992) Cell 71:973.
  3. Morrison, S.J. et al. (1999) Cell 96:737.
  4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
  5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
  6. Cassiman, D. et al. (2001) Hepatology 33:148.

    Long Name

    Nerve Growth Factor Receptor

    Alternate Names

    CD271, NGF R, p75 NTR, TNFRSF16

    Entrez Gene IDs

    4804 (Human); 18053 (Mouse); 117089 (Rat)

    Gene Symbol

    NGFR

    UniProt

    P08138

    Additional NGFR/TNFRSF16 Products

    Product Documents for Human NGFR/TNFRSF16 APC-conjugated Antibody

    Product Datasheets

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Human NGFR/TNFRSF16 APC-conjugated Antibody

    For research use only

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