Human NKG2A/CD159a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1059
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Affinity Chromatography, CyTOF-reported, ELISA Capture, Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 131411
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
Specificity
Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human NKG2A/CD159a Antibody
Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP by Flow Cytometry.
HEK293 Human embryonic kidney cell line transfected with either (A) Human NKG2A/CD159a, or (B) Human NKG2B/CD159b and eGFP was stained with Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.Applications for Human NKG2A/CD159a Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood cells and HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP
Sample: Human peripheral blood cells and HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2A/CD159a
NKG2A, also known as CD159a, is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.
Long Name
Natural Killer G2A
Alternate Names
CD159a, Klrc1, NKG2
Gene Symbol
KLRC1
Additional NKG2A/CD159a Products
Product Documents for Human NKG2A/CD159a Antibody
Product Specific Notices for Human NKG2A/CD159a Antibody
For research use only
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