Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. Nocross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human NKG2C/CD159c Antibody

Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NKG2C/CD159c Antibody

Application

Recommended Usage

CyTOF-reported

Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB1381 in the following applications:

Flow Cytometry (2 Reviews)
qPCR (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8⁺ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8⁺ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C⁺ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).

References

Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
Tassi I. et al. (2006) Immnunol Rev. 214:92.
Lanier, L.L. (2008) Nat. Immunol. 9:495.
Schleinitz, N. et al. (2010) Immunology. 174:2878.
Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
Braud, V.M. et al. (1998) Nature. 391:795.
Lanier, L.L. (1998) Immunity 8:693.
Vance, R.E. et al. (1999) J Exp Med 190:1801.
Kaiser B.K. et al. (2005) J Immunol 174:2878.
Bellón T. et al. (1999) J Immunol 162:3996.
Pupuleku A. et al. (2017) Front Immunol. 8:1317.
Hammer Q. et al. (2018) Nat Immunol. 19:453.
Alici, E. et al. (2008) Blood 111:3155.
Coupel, S. et al. (2007) Blood 109:2806.
Fausther-Bovendo, H. et al. (2008) AIDS 22:217.

Long Name

Natural Killer G2C

Alternate Names

CD159c, KLRC2, NKG2-C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Gene Symbol

KLRC2

Additional NKG2C/CD159c Products

Product Documents for Human NKG2C/CD159c Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human NKG2C/CD159c Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NKG2C/CD159c Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1381

Key Product Details

Species Reactivity

Validated:

Cited:

Applications