Human NKG2C/CD159c PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB138P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Bioassay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 134591
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Specificity
Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NKG2C/CD159c PE-conjugated Antibody
Detection of NKG2C/CD159c in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c PE-conjugated Monoclonal Antibody (Catalog # FAB138P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry
Enhancement of cytokine‐driven IFN‐ gamma production within CD56bright, CD56dimNKG2C−CD57− and CD56dimNKG2C+CD57− NK cells after vaccination. (A) Frequencies of IFN‐ gamma producing NK cells after HCC stimulation of PBMC at baseline (0) and up to 24 weeks post TIV vaccination. (B) Gating strategy showing CD57 and NKG2C‐defined subsets. (C, D) Subset distribution for NK cell IFN‐ gamma production after HCC stimulation of pre (0 weeks) and post‐vaccination (4, 12, 24 weeks) samples from subjects receiving (C) TIV and (D) DTPiP. Dots represent the frequency of IFN‐ gamma+ NK cells for each subject and horizontal lines represent median frequency for each group. Statistical analysis was performed on paired samples using Wilcoxon signed‐rank test, *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28383105), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human NKG2C/CD159c PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs)
Reviewed Applications
Read 4 reviews rated 4.3 using FAB138P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2C/CD159c
References
- Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
- Tassi I. et al. (2006) Immnunol Rev. 214:92.
- Lanier, L.L. (2008) Nat. Immunol. 9:495.
- Schleinitz, N. et al. (2010) Immunology. 174:2878.
- Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
- Braud, V.M. et al. (1998) Nature. 391:795.
- Lanier, L.L. (1998) Immunity 8:693.
- Vance, R.E. et al. (1999) J Exp Med 190:1801.
- Kaiser B.K. et al. (2005) J Immunol 174:2878.
- Bellón T. et al. (1999) J Immunol 162:3996.
- Pupuleku A. et al. (2017) Front Immunol. 8:1317.
- Hammer Q. et al. (2018) Nat Immunol. 19:453.
Long Name
Natural Killer G2C
Alternate Names
CD159c, KLRC2, NKG2-C
Gene Symbol
KLRC2
Additional NKG2C/CD159c Products
Product Documents for Human NKG2C/CD159c PE-conjugated Antibody
Product Specific Notices for Human NKG2C/CD159c PE-conjugated Antibody
For research use only
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