Human NKX2.5 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2444
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Gln24-Arg137
Accession # P52952
Specificity
Detects human NKX2.5 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NKX2.5 Biotinylated Antibody
NKX2.5 in Human Heart.
NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human NKX2.5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2444) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NKX2.5 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of human fetal brain and immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed frozen sections of human fetal brain and immersion fixed paraffin-embedded sections of human heart
Western Blot
0.1 µg/mL
Sample: Recombinant Human NKX2.5
Sample: Recombinant Human NKX2.5
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKX2.5
Long Name
NK2 Homeobox 5
Alternate Names
CSX1, NKX2-5, NKX2E
Entrez Gene IDs
1482 (Human)
Gene Symbol
NKX2-5
UniProt
Additional NKX2.5 Products
Product Documents for Human NKX2.5 Biotinylated Antibody
Product Specific Notices for Human NKX2.5 Biotinylated Antibody
For research use only
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