Human NLRP3/NALP3 (aa 540-689) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7010
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NLRP3/NALP3
Lys540-Lys689
Accession # Q96P20
Lys540-Lys689
Accession # Q96P20
Specificity
Detects human NLRP3/NALP3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) NALP1, rhNALP2, and rhNALP5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NLRP3/NALP3 (aa 540-689) Antibody
NLRP3/NALP3 in THP-1 Human Cell Line.
NLRP3/NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line, unstimulated (lower panel) or stimulated (upper panel) with 50 ng/mL PMA and 200 ng/mL LPS, using Goat Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7010) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human NLRP3/NALP3 (aa 540-689) Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line, stimulated with 50 ng/mL PMA and 200 ng/mL LPS
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line, stimulated with 50 ng/mL PMA and 200 ng/mL LPS
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP3/NALP3
NALP3 (NAcht, Leucinerichrepeat and PYD domain containing protein 3; also PYPAF1, CIAS1 and CLR1.1) is a cytoplasmic 115‑130 kDa member of the NLRP family of molecules. It is selectively expressed, being found in stratified squamous and transitional epithelium, Hassell's corpuscles, neutrophils, dendritic cells and T cells. NALP3 promotes IL-1 beta and IL-18 maturation by activating caspase‑1. It does so by forming a NALP3 inflammasome comprised of NALP3, ASC and caspase‑1. Bacterial RNA and cell wall peptidoglycan can bind to NALP3, promoting (potentially) ATP binding, NALP3 oligomerization, and caspase activation. Human NALP3 is 1036 amino acids (aa) in length. It contains an N-terminal DAPIN domain (aa 1-93), a NACHT domain (aa 220-536), and seven consecutive LRRs (aa 740‑991). Alternate splice forms are reported. Either individually, or in combination, there can be deletions of aa 836-892 or 721-777, and a premature truncation after Gly719 that generates a 70 kDa isoform.
Long Name
NLR Family, Pyrin Domain Containing 2
Alternate Names
AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1
Gene Symbol
NLRP3
UniProt
Additional NLRP3/NALP3 Products
Product Documents for Human NLRP3/NALP3 (aa 540-689) Antibody
Product Specific Notices for Human NLRP3/NALP3 (aa 540-689) Antibody
For research use only
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