Human Notch-1 Intracellular Domain Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3647
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Notch-1
Gly2428-Lys2556
Accession # AAG33848
Gly2428-Lys2556
Accession # AAG33848
Specificity
Detects human Notch‑1 Intracellular Domain (ICD) in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Notch-1 Intracellular Domain Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK)
Sample: Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-1
Notch-1 (so named for "notches" in fly wings; also TAN-1) is a 300 kDa member of the Notch family of glycoproteins. It is associated with gene activation in both embryo and adult. Human Notch-1 is a 2538 amino acid (aa) type I transmembrane glycoprotein. It undergoes Golgi processing to generate a heterodimer composed of a 180‑200 kDa disulfide-linked extracellular domain (aa 18‑1664) and a 120 kDa membrane-bound segment (aa 1665‑2556). Upon ligand binding, the 110 kDa segment undergoes two cleavages which generate an NICD (notch intracellular domain) (aa 1754‑2556), a nuclear transcription factor. One isoform shows a deletion of aa 248‑288. Over aa 2428‑2556, human Notch 1 is 83% and 89% aa identical to canine and mouse Notch-1, respectively.
Alternate Names
Notch1, TAN1
Gene Symbol
NOTCH1
UniProt
Additional Notch-1 Products
Product Documents for Human Notch-1 Intracellular Domain Biotinylated Antibody
Product Specific Notices for Human Notch-1 Intracellular Domain Biotinylated Antibody
For research use only
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