Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Notch-2 APC-conjugated Antibody

Detection of Notch‑2 in NTera‑2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Notch-2 APC-conjugated Monoclonal Antibody (Catalog # FAB37351A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Notch-2 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Gene Symbol

NOTCH2

UniProt

Q04721

Additional Notch-2 Products

Product Documents for Human Notch-2 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Notch-2 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Notch-2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB37351A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Notch-2 Antibodies »

Product Specifications

Immunogen

Specificity