Human Notch-4 Intracellular Domain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3847

R&D Systems, part of Bio-Techne
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MAB3847
MAB3847-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 411913

View all Notch-4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Notch‑4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466

Specificity

Detects human Notch‑4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Notch-1 Intracellular Domain, rhNotch-2 Intracellular Domain, rhNotch-3, recombinant rat (rr) Notch-1 EC,rrNotch‑2 EC, or rrDelta-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Notch-4 Intracellular Domain Antibody

Notch-4 antibody in Human HUVEC by Immunocytochemistry (ICC).

Notch‑4 in Human HUVEC.

Notch-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Notch-4 Intracellular Domain Monoclonal Antibody (Catalog # MAB3847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Notch-4 Intracellular Domain Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human Notch-4 Intracellular Domain
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using MAB3847 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-4

Notch-4 is a 250 kDa member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch-4 is a 1980 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535 aa cytoplasmic region. Notch-4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa 1310‑1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-J kappa and activates genes associated with HES-1. Over cytoplasmic aa 1778‑2003, human Notch‑4 is 78% aa identical to mouse Notch-4.

Alternate Names

INT3, Notch4

Entrez Gene IDs

4855 (Human); 18132 (Mouse); 406162 (Rat)

Gene Symbol

NOTCH4

UniProt

Q99466

Additional Notch-4 Products

Product Documents for Human Notch-4 Intracellular Domain Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Notch-4 Intracellular Domain Antibody

For research use only

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