Human NQO-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7567
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Xenograft
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 844142
Product Specifications
Immunogen
E. coli-derived recombinant human NQO-1
Met1-Lys274
Accession # P15559
Met1-Lys274
Accession # P15559
Specificity
Detects human NQO-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human NQO-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NQO-1 Antibody
Detection of Human NQO-1 by Western Blot.
Western blot shows lysates of human lung tissue, human kidney tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NQO-1 Monoclonal Antibody (Catalog # MAB7567) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NQO-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NQO-1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with recombinant human NQO-1, see our available Western blot detection antibodies.
Sample: Cell lysates spiked with recombinant human NQO-1, see our available Western blot detection antibodies.
Western Blot
0.5 µg/mL
Sample: Human lung tissue, human kidney tissue, and MCF‑7 human breast cancer cell line
Sample: Human lung tissue, human kidney tissue, and MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NQO-1
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Entrez Gene IDs
1728 (Human)
Gene Symbol
NQO1
UniProt
Additional NQO-1 Products
Product Documents for Human NQO-1 Antibody
Product Specific Notices for Human NQO-1 Antibody
For research use only
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