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Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3771

R&D Systems, part of Bio-Techne
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MAB3771-100
MAB3771-500
MAB3771-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 147705

Product Specifications

Immunogen

E. coli-derived recombinant human NRG1-beta 1/HRG1-beta 1
Ser2-Lys246
Accession # AAA58639

Specificity

Detects human NRG1-beta 1 in ELISAs. Recognizes an epitope found in the EGF-like domain of NRG1‑ beta1 (amino acids 179‑246). It also detects recombinant human SMDF (Sensory and Motor neuron-Derived Factor), but it does not recognize the EGF-like domain of human NRG1-alpha (amino acids 177‑241). The epitope is present within amino acids 213‑230 of human NRG1-beta 1. This segment is present in all NRG1 beta isoforms, GGF‑2 (glial growth factor), and SMDF, but it is absent from NRG1-alpha, NDF43, and NRG1-gamma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

Application
Recommended Usage

Human NRG1-beta 1/HRG1-beta 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain Biotinylated Antibody (Catalog # BAF377)
  • Standard: Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein (Catalog # 377-HB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1 beta 1/NRG1 beta 1

Neuregulin-1, also known as Heregulin 1, functions as a ligand for the receptor tyrosine kinases, ErbB3 and ErbB4. It exists in several different mRNA splicing variants resulting in both transmembrane and secreted forms.

Alternate Names

Neuregulin1 beta 1

Entrez Gene IDs

3084 (Human); 211323 (Mouse); 112400 (Rat)

Gene Symbol

NRG1

Additional Neuregulin-1 beta 1/NRG1 beta 1 Products

Product Documents for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

For research use only

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