Human NTH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2675
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NTH1
Met1-Leu312
Accession # P78549
Met1-Leu312
Accession # P78549
Specificity
Detects human NTH1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NTH1 Antibody
Detection of Human NTH1 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human NTH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NTH1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human NTH1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NTH1
NTH1 (Endonuclease III) is a DNA glycosylase with AP lyase activity. It functions as an enzyme to repair oxidative damage to DNA through the BER (base excision repair) pathway.
Long Name
Nth Endonuclease III-like 1
Alternate Names
NTHL1, OCTS3
Entrez Gene IDs
4913 (Human)
Gene Symbol
NTHL1
UniProt
Additional NTH1 Products
Product Documents for Human NTH1 Antibody
Product Specific Notices for Human NTH1 Antibody
For research use only
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