Human Nucleoporin NUP85 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5976
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NUP85
Accession # Q9BW27
Accession # Q9BW27
Specificity
Detects human NUP85 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NUP85 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Nucleoporin NUP85 Antibody
Detection of Human Nucleoporin NUP85 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Nucleoporin NUP85 in Human MSCs.
Nucleoporin NUP85 was detected in immersion fixed human multipotent stromal cells (MSCs) using Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF5976) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Nucleoporin NUP85 in HeLa Human Cell Line.
Nucleoporin NUP85 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Nucleoporin NUP85 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells, human multipotent stromal cells (MSCs), and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human mesenchymal stem cells, human multipotent stromal cells (MSCs), and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nucleoporin NUP85
Long Name
Nuclear Pore Complex Protein Nup85
Alternate Names
FROUNT, Nup75, NUP85, PCNT1, Pericentrin-1
Gene Symbol
NUP85
UniProt
Additional Nucleoporin NUP85 Products
Product Documents for Human Nucleoporin NUP85 Antibody
Product Specific Notices for Human Nucleoporin NUP85 Antibody
For research use only
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