Human Numb Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF4338

R&D Systems, part of Bio-Techne
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BAF4338
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AF4338
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all Numb Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Numb
Thr356-Leu592
Accession # AAH68476

Specificity

Detects human Numb in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Numb Biotinylated Antibody

Numb in RT-4 Human Cell Line.

Numb was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line using Sheep Anti-Human Numb Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Numb Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Numb
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Numb

Numb is a member of a family of cytoplasmic adaptor proteins termed CLASPS (Clathran-associated Sorting Proteins). These link transmembrane cargo proteins to the endocytic machinery. Numb, a conserved cell-fate determining factor, is normally associated with the basolateral surface of polarized cells. Movement towards the apical surface induces its phosphorylation, which precludes its entry into this region. Human Numb is 651 amino acids (aa) in length. It contains one PTB (aa 24‑172) and one PRR (aa 366‑413) domain. There is a potential for multiple alternate splice forms that involve all regions of the molecule. Over aa 356‑592, human Numb shares 88% and 90% aa identity with mouse and canine Numb, respectively.

Alternate Names

m-numb, mnb, S171

Entrez Gene IDs

8650 (Human); 18222 (Mouse); 29419 (Rat)

Gene Symbol

NUMB

UniProt

AAH68476

Additional Numb Products

Product Documents for Human Numb Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Numb Biotinylated Antibody

For research use only

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