HEK293 human embryonic kidney cell line transfected with (A) human OCTN1/SLC22A4 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN1/SLC22A4 Monoclonal Antibody (Catalog # MAB10985) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human OCTN1/SLC22A4 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human OCTN1/SLC22A4 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCTN1/SLC22A4

Polyspecific organic cation transporters are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A4 is an organic cation transporter and integral plasma membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by SLC22A4 is at least partially ATP-dependent. SLC22A4 may play a role in Crohn's disease.

Long Name

Solute Carrier Family 22 (Organic Cation/Ergothioneine Transporter) Member 4

Alternate Names

ETT, SLC22A4, UT2H

Entrez Gene IDs

6583 (Human); 30805 (Mouse)

Gene Symbol

SLC22A4

UniProt

Q9H015

Additional OCTN1/SLC22A4 Products

Product Documents for Human OCTN1/SLC22A4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human OCTN1/SLC22A4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human OCTN1/SLC22A4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10985

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all OCTN1/SLC22A4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human OCTN1/SLC22A4 Antibody

Detection of OCTN1/SLC22A4 in HEK293 Human Cell Line Transfected with Human OCTN1/SLC22A4 and eGFP by Flow Cytometry.