HEK293 human embryonic kidney cell line transfected with (A) human OCTN2/SLC22A5 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN2/SLC22A5 Monoclonal Antibody (Catalog # MAB11004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human OCTN2/SLC22A5 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCTN2/SLC22A5

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for the elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A5 is an integral plasma membrane protein which functions as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. Mutations in SLC22A5 are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.

Long Name

Solute Carrier Family 22 (Organic Cation/Carnitin Transporter) Member 5

Alternate Names

CDSP, OCTN2VT, SCD, SLC22A5

Entrez Gene IDs

6584 (Human); 20520 (Mouse); 29726 (Rat)

Gene Symbol

SLC22A5

UniProt

O76082 (Human)

Additional OCTN2/SLC22A5 Products

Product Documents for Human OCTN2/SLC22A5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human OCTN2/SLC22A5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human OCTN2/SLC22A5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11004

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all OCTN2/SLC22A5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human OCTN2/SLC22A5 Antibody

Detection of OCTN2/SLC22A5 in HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP by Flow Cytometry