Human Osteocalcin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB113272
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 582014
Product Specifications
Immunogen
Osteocalcin/KLH containing peptide
Accession # P02818
Accession # P02818
Specificity
Detects human Osteocalcin in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Osteocalcin Antibody
Detection of Osteocalcin in SAOS-2 cells by Flow Cytometry.
SAOS-2 cells were stained with Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB113272, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.Applications for Human Osteocalcin Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: SAOS-2 cells fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).
Sample: SAOS-2 cells fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteocalcin
References
- Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Gene Symbol
BGLAP
UniProt
Additional Osteocalcin Products
Product Documents for Human Osteocalcin Antibody
Product Specific Notices for Human Osteocalcin Antibody
For research use only
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