Detects human Osteopontin/OPN in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse Osteopontin/OPN.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Osteopontin/OPN PE-conjugated Antibody

Detection of Osteopontin/OPN in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Osteopontin/OPN PE-conjugated Monoclonal Antibody (Catalog # IC14331P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Osteopontin/OPN PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: U937 human histiocytic lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Osteopontin/OPN

Osteopontin (OPN), previously referred to as Transformation-associated Secreted Phosphoprotein, Bone Sialoprotein I, 2ar, 2B7, Early T Lymphocyte Activation 1 Protein, Minopotin and Calcium Oxalate Crystal Growth Inhibitor Protein, is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the Integrins alpha_v beta₁, alpha_v beta₃, alpha_v beta₅, and non-RGD-mediated interaction with CD44 and the Integrins alpha₈ beta₁ or alpha₉ beta₁. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by Thrombin, Enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References

Ann. N.Y. Acad. Sci. (1995) 760, Apr. 21.
Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Gene Symbol

SPP1

UniProt

NP_000573.1

Additional Osteopontin/OPN Products

Product Documents for Human Osteopontin/OPN PE-conjugated Antibody

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COA

SDS

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Product Specific Notices for Human Osteopontin/OPN PE-conjugated Antibody

For research use only

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Flow Cytometry

Human Osteopontin/OPN PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC14331P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity