Human Osteoprotegerin/TNFRSF11B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8051
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Antibody Array Development, ELISA Development, ELISA Development (Capture), Immunocytochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 69127
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
Glu22-Leu401
Accession # O00300
Glu22-Leu401
Accession # O00300
Specificity
Detects human Osteoprotegerin/TNFRSF11B in ELISAs. It has been reported (1), and R&D Systems testing has confirmed, that this antibody shows strong cross-reactivity with an unrelated protein, human Carbonic Anhydrase II. In Western blots, this antibody does not cross‑react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD30, rmCD40, rhDR3, rhDR6, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLT betaR, rhNGF R, rmOPG, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Osteoprotegerin/TNFRSF11B Antibody
Application
Recommended Usage
Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB8051 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B
Osteoprotegerin is a member of the TNF receptor superfamily expressed by many cell types. It exists in soluble monomeric and dimeric forms as a decoy receptor for its ligands, TRANCE and TRAIL. Osteoprotegerin functions as an antagonist to RANKL-induced osteoclastogenesis and bone resorption.
References
- Waterman, E.A. et al. (2007) Intl. J. Cancer 121:1958.
Alternate Names
OCIF, TNFRSF11B
Gene Symbol
TNFRSF11B
UniProt
Additional Osteoprotegerin/TNFRSF11B Products
Product Documents for Human Osteoprotegerin/TNFRSF11B Antibody
Product Specific Notices for Human Osteoprotegerin/TNFRSF11B Antibody
For research use only
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