Human P2X7/P2RX7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9790
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 545230
Product Summary for Human P2X7/P2RX7 Antibody
Immunogen
E. coli-derived recombinant human P2X7/P2RX7
Asp356-Tyr595
Accession # Q99572
Asp356-Tyr595
Accession # Q99572
Specificity
Detects human P2X7/P2RX7 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human P2X7/P2RX7 Antibody
P2X7/P2RX7 in WM‑115 Human Cell Line.
P2X7/P2RX7 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB9790) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human P2X7/P2RX7 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed WM‑115 human malignant melanoma cell line
Sample: Immersion fixed WM‑115 human malignant melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: P2X7/P2RX7
Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.
Long Name
P2X Purinergic Receptor 7
Alternate Names
P2RX7
Gene Symbol
P2RX7
UniProt
Additional P2X7/P2RX7 Products
Product Documents for Human P2X7/P2RX7 Antibody
Product Specific Notices for Human P2X7/P2RX7 Antibody
For research use only
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