Human p38 beta/MAPK11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3274

R&D Systems, part of Bio-Techne
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MAB3274
MAB3274-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 280071

View all p38 beta/MAPK11 Antibodies »

Product Specifications

Immunogen

Human p38 beta/MAPK11 synthetic peptide
EPPKPPGSLEIEQ
Accession # NP_002742

Specificity

Detects human p38 beta/MAPK11 in direct ELISAs. No cross-reactivity with recombinant human p38 alpha, gamma, or delta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human p38 beta/MAPK11 Antibody

p38 beta/MAPK11 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

p38 beta/MAPK11 in Jurkat Human Cell Line.

p38 beta/MAPK11 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta/MAPK11 Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human p38 beta/MAPK11 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 beta/MAPK11

The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38 delta, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.

Long Name

Mitogen-Activated Protein Kinase 11

Alternate Names

MAPK11, MPK11, p38b, p38beta, PRKM11, SAPK2B

Entrez Gene IDs

5600 (Human); 19094 (Mouse); 689314 (Rat)

Gene Symbol

MAPK11

UniProt

NP_002742

Additional p38 beta/MAPK11 Products

Product Documents for Human p38 beta/MAPK11 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human p38 beta/MAPK11 Antibody

For research use only

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